Fish Pathogen Monitoring Studies
Fish Pathogen Monitoring Studies
Ceratonova shasta is a freshwater, myxozoan parasite that is native to the Pacific North West of North America. It causes enteronecrosis in juvenile salmonids and is associated with population-level impacts in the Klamath River. Transmission occurs through waterborne stages: actinospores released from annelids infect salmonid fishes and develop into myxospores which then infect annelids (see life cycle, above). The parasite proliferates in each host.
In response to the high prevalence and severity of C. shasta-infection in Klamath salmonids, we developed a parasite monitoring program to track the spatial and temporal abundance of C. shasta. The three main approaches are based on the parasite's life cycle and include sentinel fish exposures, annelid host sampling and molecular quantification of parasite DNA in water samples. These are described in more detail below. Monitoring occurs at established index sites which are shown on the following map.
Data shared here are preliminary and subject to modification.
Page photo credits: S Atkinson, S Hallett & J Alexander
Monitoring Studies are Primarily Supported by the Bureau of Reclamation.
Sentinel fish exposures
In sentinel fish exposures, fish highly susceptible to the parasite (out-of-basin rainbow trout) are placed in cages alongside fish of interest such as in-basin Chinook and coho salmon at index sites along the river for a three day exposure. All fishes are transported to OSU's John L Fryer Aquatic Animal Health Lab and monitored for infection (~ 60 days). Severity of infection (percent morbidity and mean days to morbidity) are recorded through visual observations and molecular assay (PCR).
2024 data updates
An April sentinel fish exposure is scheduled for 04/18 - 04/21 at KBC and KSV with Fall Creek Hatchery Fall Chinook and out-of-basin rainbow trout.
A May sentinel fish exposure will occur 05/20 - 05/23 at WMR and KED in the upper basin and at KI5, KBC, KSV and KOR in the lower basin.
A third sentinel fish exposure is scheduled to occur at all index sites in June, 2024.
An exposure is scheduled to occur in September at two lower basin sites.
Water samples
To detect and quantify waterborne stages of C. shasta, river water samples are collected at five mainstem index sites; once a week all year round at two sites (KBC and KSV) and once per week from April through October at three other sites. Solar-powered automatic samplers (ISCOs) collect 1L water every 2 hours for 24 hours, from which 4 1L samples are manually taken (see photos below). Each 1L sample is filtered through a nitrocellulose membrane using a vacuum pump, and any captured DNA in 3 of the replicate samples is extracted using a kit (see images on right). A quantitative PCR (qPCR) specific for C. shasta is used to detect and quantify any parasite DNA present. Cq values generated by the qPCR are converted to numbers of parasite spores per liter of water using reference samples with known quantities of spores. The Karuk and Yurok tribal biologists are integral to the collection and filtration of the water samples. Water samples are also taken in conjunction with the sentinel fish exposures; manual 'grab' samples are collected on the first and last day of the exposure. Data are presented as the average spores per liter of three replicate 1 liter water samples collected at each site and time.
Genotyping
There are multiple genetic types or genotypes of C. shasta simultaneously present in the Klamath River. These differentially disease the various salmonid species. For example, type I causes mortality in Chinook salmon whereas type II can be fatal for coho salmon. Type 0 is found in sympatric Oncorhynchus mykiss (steelhead and redband rainbow trout).
Therefore, we also genotype each water sample. This is done in two ways. For genotyping type II only, we use a qPCR that amplifies the variable ITS1 region specific to that genotype. Whereas, to determine the overall genotype composition of a sample, we amplify the variable ITS1 region common to all genotypes in a PCR and then sequence that amplicon. From the sequencing chromatogram, we can determine the proportion of each genotype present in a sample. We use the total spore density to then determine the number of spores of each genotype in a sample.
Genotyping commences once we detect greater than 1 spore per liter.
2024 data updates
We are still in the Winter collection phase, with only two Klamath River sites sampled each week: KSV & KBC. OSU has received and analyzed water samples up to and including 2/26/2024.
Samples were taken either by ISCO automatic sampler "i" and are given as the average density of C. shasta spores per liter of river water (from 3x 1-L subsamples of a 24-hour composite sample, Sunday-Monday), or taken manually "g" (3 x 1-L samples taken Monday).
No C. shasta DNA has been detected in water, which is not unusual during winter.
Specific results for 2 sites, '-' = no C. shasta DNA detected. If inhibition was detected this is specified. At the end of January and early February the high suspended sediment load caused inhibition of the molecular assay.
01/02/2023
KBC -
KSV -
01/08/2023
KBC - (slight inhibition)
KSV no sample
01/15/2023
KBC - (slight inhibition)
KSV - (totally inhibited)
01/22/2023
KBC - (no inhibition)
KSV - (no inhibition)
01/29/2023
No samples - sediment load too high
02/05/2023
No samples - sediment load too high
02/12/2023
No samples - sediment load too high
02/19/2023
KBC -
KSV -
02/26/2023
KBC -
KSV -
C. shasta DNA was detected in only one subsample collected at KBC and KSV during these three weeks.
Specific results for 2 sites, '-' = no C. shasta DNA detected. If inhibition was detected this is specified.
Sampling at all index sites will commence late March.
03/04/2024
KBC trace (in 1/3 samples)
KSV -
03/12/2024
KBC -
KSV -
03/18/2024
KBC -
KSV -
This was the first week of sampling at all 6 index sites. Multiple sites required double the regular number of filters due to high levels of suspended material.
No C. shasta DNA was detected at any site this week.
03/25/2024
KSH - (substitute site for KI5)
KBC -
KMN -
KSV -
KOR -
KTC -
Due to the higher than usual amount of suspended material in the water column, which renders filtering challenging, less water was filtered at most sites this week (volume indicated). ISCO = automatic water sampler; grab = manual sample. '-' = no C. shasta DNA detected
No C. shasta DNA detected was detected at any site this week, however, the sensitivity of the approach is affected by the high river turbidity.
04/01/2024
KSH - (100ml; grab)
KBC - (100ml; isco)
KMN - (100ml; grab)
KSV - (100ml; isco)
KOR - (250ml; isco)
KTC - (1L; grab)
This was the first week of sampling at all index sites, with expedited sample processing. Multiple sites required a reduction in water volume filtered due to high levels of suspended material. Two sites were sampled with an automatic sampler ("i"), four sites were a manual grab ("grab")(water levels were too high at KTC to deploy an ISCO).
Only trace amounts of Cs-DNA was detected at any of the 6 sites analyzed (at KTC):
04/08/2024
KSH - (100ml; isco)
KBC - (100ml; grab)
KMN trace (1/3 samples positive; 100ml; isco)
KSV - (100ml; grab)
KOR - (250ml; grab)
KTC - (1L; grab)
This was the 2nd week of sampling at all index sites, with expedited sample processing. C. shasta DNA was detected at 5 of the 6 index sites (in at least one of three replicate samples). Because of the higher than usual levels of suspended substrate, subsamples of less than 1L (100 - 350mL) were filtered for each site except the lowermost, KTC.
Estimated spores/L based on low-volume subsamples (reduced owing to ongoing high sediment loads):
04/15/2024
KSH 2/3 samples positive; 100mL; isco; estimated 3 sp/L
KBC 2/3 samples positive; 100mL; isco; estimated 6 sp/L
KMN 3/3 samples positive; 100mL; isco; estimated 5 sp/L
KSV 1/3 samples positive; 100mL; grab; estimated <1 sp/L
KOR 3/3 samples positive; 350mL; isco; estimated 4 sp/L
KTC 0/3 samples positive; 1L; grab; negative
We will test 1 replicate sample with > 5 spores/L from each site for genotype II (coho-relevant).
Annelid sampling
To monitor abundance and prevalence of C. shasta infection in the invertebrate annelid host, Manayunkia occidentalis, benthic samples are collected annually at seven sites in the Klamath River. Sites span a discharge gradient; 2 are located in the upper basin downstream from Keno Dam, 3 are located in the middle basin downstream from Iron Gate Dam, and 2 are located in the lower basin downstream from the Scott River. Samples are routinely collected in fall, winter, spring, and summer, and are scheduled to occur more frequently if flooding or pulse flow events are planned. Samples are processed to determine density, simple demographics, and prevalence of C. shasta infection.
Annual reports
Annual reports for Bureau of Reclamation funded studies for 2015 onwards are available. Please contact Sascha Hallett (halletts@oregonstate.edu). Annual reports are submitted June 1 the year after the research year.
Annual Klamath River fish health workshop
We are pleased to announce this year's Annual Klamath River Fish Health Workshop to be held in Yreka, May 17th & 18th. We gratefully acknowledge our Karuk collaborators for hosting us at their Karuk Tribe Housing Authority, Yreka Apsuun Community Center.
This year, the KRFHW will follow the Spring KBMP meeting to be held Tuesday May 16th at the same venue (please see emails from Randy Turner for KBMP specifics).
The general KRFHW schedule will be as follows:
Workshop: Wednesday May 17th (9am-5pm). Full day, public forum during which Klamath River fish health research updates, overviews, and presentations will be shared with an open forum Q & A session. Presentations will be basin-wide. Plans for 2023 and beyond will also be shared. The May 17 workshop will be open to the public to promote an open exchange of information, and attendance for all is free.
Project Planning Session: Thursday May 18th (9am-12pm). Half day, smaller meeting to coordinate research efforts and management needs. The Project planning session is reserved for fish health researchers to discuss 2023 and beyond study plans and to promote project coordination and collaboration. The planning session will include an opportunity for Q & A between fish health researchers and agency/tribal co-managers.
The meeting format will be hybrid, with in-person attendance highly recommended but in the spirit of inclusivity and accessibility, participants will also be able to connect via online (link will be provided following registration).
There is no registration fee for participation, however, we do request that you register your intention to attend on this spreadsheet.
Call for KRFHW presenters: If you would like to present a research update or overview at the Workshop (May 17 public forum), please send us your research abstract (with title, presenter, co-authors, and summary of research) by Friday April 28th.
We look forward to seeing many familiar faces at this annual workshop on fish health related research in the Klamath River Basin!